its possible somethng is wrong with that cylinder
I would suspect a code for a spark error if it was a coil/wire/plug issue (mis fire)
You may want to replace your injectors now or at least service them if you have high miles
The change in color from port to port is kind of common on the OHV engines, the rear two cylinders do not get oiled as well as the front 4 in my experience, but if the change is drastic only over one port you are correct to assume a problem
It may have also been an air leak in the intake manifold gasket.
The pattern of oil/film iside the manifold is also effected greatly by the location of the vacuum ports, IAC valve, EGR valve, PCV and throttle body
What I am getting at is you may have an issue and you may not. The older metal intake manifolds I have seen off of OHV engines are not coated with oil/carbon/ film uniformly, the rear two cylinder ports are typically not as dark as the others if I remember correctly or vise versa
regardless now is your chance to check your valves, re-seal and replace the injectors, check the plugs and wires and all teh gaskets and re-seal the top of your engine properly.
Higher mileage OHV's with metal intakes develop alot of leaks in time in the lower and upper intakes, re-sealing them is a great way to get some power back and I consider it to be routine if you have over 150K miles