if anyone is running ported or sealed make certain you are running proper polarity as marked by the amp and the speakers. i'm not talking about both running + and - so they sound in phase with each other, i'm talking about being in phase with the box. this really affects ported boxes that are tuned correctly because there is an audible difference in SPL when you push and pull at the correct time WITH THE BOX, not each other. it primarily affects the ported boxes more because they shift phase and allow the front and the backwave to collide.
everyone agree's that airspace in a box is critical, right?
ok, have you ever seen a sub on a sine sweep bias itself outward on certain frequencies (specifically lower freq's)? (if you haven't, go look, it will) This is what happens when DC get's in the signal. Certain higher frequencies induce a DC effect, as do lower one's. certain amps simply don't filter well and allow dc in the signal path. By running the sub(s) opposite of the polarity the amp and sub state, you are firing the bias into the car instead of the box.
Now if airspace is critical inside a box, firing the wave out of the box rather than into the box means that you are using the car side of the box rather than the chamber side for certain resonant frequencies. This decreases SPL on certain notes.
If you want a little test, reverse your leads on your amp (not one channel, all of them) and play a few bassy, yet overall challenging, songs at mid volume, then upper volume. you will notice a difference at the frequency the box is tuned at. On some it won't be terribly obvious, on others it could mean a db or two.
The most common comment on rephasing the amp is "i get a little better/deeper low tone's".
Give it a shot if you are not certain your subs and amp are perfectly inline with each other. Proper phase of the speaker makes all the difference in the world, but proper phase of the sub and amp to the box can really bring out the potential in your system.
Good luck!